ICAM-1 targeted catalase encapsulated PLGA-b-PEG nanoparticles against vascular oxidative stress

J Microencapsul. 2015;32(7):687-98. doi: 10.3109/02652048.2015.1073384. Epub 2015 Aug 31.

Abstract

Targeted delivery of therapeutics is the favourable idea, whereas it is possible to distribute the therapeutically active drug molecule only to the site of action. For this purpose, in this study, catalase encapsulated poly(D,L-lactide-co-glycolide)-block-poly(ethylene glycol) (PLGA-b-PEG) nanoparticles were developed and an endothelial target molecule (anti-ICAM-1) was conjugated to this carrier system in order to decrease the oxidative stress level in the target site. According to the enzymatic activity results, initial catalase activity of nanoparticles was increased from 27.39 U/mg to up to 45.66 U/mg by adding 5 mg/mL bovine serum albumin (BSA). After 4 h, initial catalase activity was preserved up to 46.98% while free catalase retained less than 4% of its activity in proteolytic environment. Furthermore, FITC labelled anti-ICAM-1 targeted catalase encapsulated nanoparticles (anti-ICAM-1/CatNPs) were rapidly taken up by cultured endothelial cells and concomitantly endothelial cells were resistant to H2O2 induced oxidative impairment.

Keywords: Catalase; ICAM-1; PLGA-b-PEG nanoparticle; oxidative stress; vascular targeting.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Blood Vessels / drug effects*
  • Blood Vessels / metabolism
  • Catalase / administration & dosage
  • Catalase / pharmacology*
  • Cells, Cultured
  • Dogs
  • Drug Compounding
  • Endothelial Cells / drug effects
  • Excipients
  • Hydrogen Peroxide / pharmacology
  • Intercellular Adhesion Molecule-1 / drug effects*
  • L-Lactate Dehydrogenase / metabolism
  • Malondialdehyde / metabolism
  • Nanoparticles
  • Oxidative Stress / drug effects*
  • Particle Size
  • Polyesters / chemistry*
  • Polyethylene Glycols / chemistry*

Substances

  • Antioxidants
  • Excipients
  • Polyesters
  • methoxypolyethyleneglycol-poly(lactic-co-glycolic acid)
  • Intercellular Adhesion Molecule-1
  • Polyethylene Glycols
  • Malondialdehyde
  • Hydrogen Peroxide
  • L-Lactate Dehydrogenase
  • Catalase