Simple column-switching ion chromatography method for determining eight monosaccharides and oligosaccharides in honeydew and nectar

Food Chem. 2016 Mar 1;194:555-60. doi: 10.1016/j.foodchem.2015.08.049. Epub 2015 Aug 14.

Abstract

Honeydew is excreted by aphids as a sweet waste and nectar is floral honey. Honeydew and nectar are complicated samples which consist of various sugars and amino acids. In this work, a simple ion chromatography with column-switching method was developed for the simultaneous analysis of 8 monosaccharides and oligosaccharides in honeydew and nectar. A reversed-phase column was used as a pretreatment column to eliminate organics on-line and sugars were eluted from a collection loop to analytical column by using column-switching technique. This method showed good linearity (r⩾0.9994) and afforded low limits of detection ranging from 1.55 to 10.17μgL(-1) for all the analytes. Recoveries ranged from 95% to 105% and repeatability results were acceptable with relative standard deviation of less than 3.21% (n=6). This method was successfully applied to quantification of these sugars in honeydew and nectar. These results showed honeydew had much more oligosaccharides than nectar.

Keywords: Column-switching; Honeydew; Ion chromatography; Nectar; Sugars.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrates / analysis
  • Chromatography, High Pressure Liquid / methods*
  • Fruit / chemistry*
  • Monosaccharides / analysis
  • Monosaccharides / chemistry*
  • Oligosaccharides / analysis
  • Oligosaccharides / chemistry*
  • Plant Nectar / analysis*

Substances

  • Carbohydrates
  • Monosaccharides
  • Oligosaccharides
  • Plant Nectar