Antioxidant and anti-ageing activities of citrus-based juice mixture

Food Chem. 2016 Mar 1;194:920-7. doi: 10.1016/j.foodchem.2015.08.094. Epub 2015 Aug 24.


The production of excessive reactive oxygen species by exposure to oxidative stress and solar radiation are primary factors in skin damage. We examined the effects of a citrus-based juice mixture and its bioactive compounds on antioxidant and anti-ageing activities in human dermal fibroblasts and hairless mice via the regulation of antioxidant enzymes and the mitogen-activated protein kinase pathway. The citrus-based juice mixture reduced H2O2-induced cell damage and intracellular reactive oxygen species production in human dermal fibroblasts. Citrus-based juice mixture pretreatment suppressed the activation of the H2O2-mediated mitogen-activated protein kinase pathway by activating the expression of activator protein 1 and matrix metalloproteinases. Moreover, it increased the expression levels of antioxidant enzymes such as glutathione reductase, catalase and manganese superoxide dismutase. In addition, oral administration of the citrus-based juice mixture decreased skin thickness and wrinkle formation and increased collagen content on an ultraviolet light B-exposed hairless mouse. These results indicate that the citrus-based juice mixture is a potentially healthy beverage for the prevention of oxidative stress-induced premature skin ageing.

Keywords: Anti-ageing; Antioxidant; Citrus-based juice mixture; Hairless mice; Human dermal fibroblasts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants
  • Citrus / chemistry*
  • Humans
  • Hydrogen Peroxide / metabolism
  • Male
  • Mice
  • Mice, Hairless
  • Oxidative Stress
  • Reactive Oxygen Species / metabolism
  • Skin Aging


  • Antioxidants
  • Reactive Oxygen Species
  • Hydrogen Peroxide