Antioxidant and anti-ageing activities of citrus-based juice mixture

Food Chem. 2016 Mar 1;194:920-7. doi: 10.1016/j.foodchem.2015.08.094. Epub 2015 Aug 24.

Abstract

The production of excessive reactive oxygen species by exposure to oxidative stress and solar radiation are primary factors in skin damage. We examined the effects of a citrus-based juice mixture and its bioactive compounds on antioxidant and anti-ageing activities in human dermal fibroblasts and hairless mice via the regulation of antioxidant enzymes and the mitogen-activated protein kinase pathway. The citrus-based juice mixture reduced H2O2-induced cell damage and intracellular reactive oxygen species production in human dermal fibroblasts. Citrus-based juice mixture pretreatment suppressed the activation of the H2O2-mediated mitogen-activated protein kinase pathway by activating the expression of activator protein 1 and matrix metalloproteinases. Moreover, it increased the expression levels of antioxidant enzymes such as glutathione reductase, catalase and manganese superoxide dismutase. In addition, oral administration of the citrus-based juice mixture decreased skin thickness and wrinkle formation and increased collagen content on an ultraviolet light B-exposed hairless mouse. These results indicate that the citrus-based juice mixture is a potentially healthy beverage for the prevention of oxidative stress-induced premature skin ageing.

Keywords: Anti-ageing; Antioxidant; Citrus-based juice mixture; Hairless mice; Human dermal fibroblasts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants
  • Citrus / chemistry*
  • Humans
  • Hydrogen Peroxide / metabolism
  • Male
  • Mice
  • Mice, Hairless
  • Oxidative Stress
  • Reactive Oxygen Species / metabolism
  • Skin Aging

Substances

  • Antioxidants
  • Reactive Oxygen Species
  • Hydrogen Peroxide