Meta-Analysis: Effects of Probiotic Supplementation on Lipid Profiles in Normal to Mildly Hypercholesterolemic Individuals

PLoS One. 2015 Oct 16;10(10):e0139795. doi: 10.1371/journal.pone.0139795. eCollection 2015.


Introduction: Recent experimental and clinical studies have suggested that probiotic supplementation has beneficial effects on serum lipid profiles. However, there are conflicting results on the efficacy of probiotic preparations in reducing serum cholesterol.

Objective: To evaluate the effects of probiotics on human serum lipid levels, we conducted a meta-analysis of interventional studies.

Methods: Eligible reports were obtained by searches of electronic databases. We included randomized, controlled clinical trials comparing probiotic supplementation with placebo or no treatment (control). Statistical analysis was performed with Review Manager 5.3.3. Subanalyses were also performed.

Results: Eleven of 33 randomized clinical trials retrieved were eligible for inclusion in the meta-analysis. No participant had received any cholesterol-lowering agent. Probiotic interventions (including fermented milk products and probiotics) produced changes in total cholesterol (TC) (mean difference -0.17 mmol/L, 95% CI: -0.27 to -0.07 mmol/L) and low-density lipoprotein cholesterol (LDL-C) (mean difference -0.22 mmol/L, 95% CI: -0.30 to -0.13 mmol/L). High-density lipoprotein cholesterol and triglyceride levels did not differ significantly between probiotic and control groups. In subanalysis, long-term (> 4-week) probiotic intervention was statistically more effective in decreasing TC and LDL-C than short-term (≤ 4-week) intervention. The decreases in TC and LDL-C levels with probiotic intervention were greater in mildly hypercholesterolemic than in normocholesterolemic individuals. Both fermented milk product and probiotic preparations decreased TC and LDL-C levels. Gaio and the Lactobacillus acidophilus strain reduced TC and LDL-C levels to a greater extent than other bacterial strains.

Conclusions: In conclusion, this meta-analysis showed that probiotic supplementation could be useful in the primary prevention of hypercholesterolemia and may lead to reductions in risk factors for cardiovascular disease.

Publication types

  • Meta-Analysis
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cholesterol, LDL / blood*
  • Dietary Supplements*
  • Female
  • Humans
  • Hypercholesterolemia / blood*
  • Hypercholesterolemia / prevention & control*
  • Male
  • Probiotics / therapeutic use*
  • PubMed
  • Randomized Controlled Trials as Topic


  • Cholesterol, LDL

Grant support

This work was supported by Japan Society for the Promotion of Science Kakenhi Grant No. 90396391; a Grant-in-Aid for Scientific Research (C) in Japan; and by the Nagai Foundation, Tokyo, in Japan.