Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses

Viruses. 2015 Oct 13;7(10):5319-27. doi: 10.3390/v7102873.


Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography-computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.

Keywords: influenza virus; multi-modal imaging; multiplicity reactivation; reporter virus.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents / therapeutic use
  • Disease Models, Animal
  • Female
  • Genes, Reporter
  • Influenza A virus / growth & development*
  • Luminescent Measurements / methods
  • Mice, Inbred BALB C
  • Multimodal Imaging / methods*
  • Optical Imaging / methods
  • Orthomyxoviridae Infections / drug therapy
  • Orthomyxoviridae Infections / pathology*
  • Orthomyxoviridae Infections / virology*
  • Staining and Labeling / methods
  • Treatment Outcome
  • Viral Load
  • Whole Body Imaging / methods*


  • Antiviral Agents