Belliella buryatensis sp. nov., isolated from alkaline lake water

Int J Syst Evol Microbiol. 2016 Jan;66(1):137-143. doi: 10.1099/ijsem.0.000682. Epub 2015 Oct 15.

Abstract

Two bacterial isolates from water of the alkaline brackish Lake Solenoe (Buryatia, Russia), 2C and 5CT, were characterized by using a polyphasic taxonomic approach. The strains were small, non-motile, Gram-stain-negative rods that formed small orange-red colonies on the surface of marine agar. Studies based on 16S rRNA gene sequences showed that the strains were related closely to Belliella pelovolcani CC-SAL-25T (98.7 % sequence similarity). The G+C content of the DNA was 38-40 mol%. DNA-DNA hybridization values between strains 2C and 5CT and B. pelovolcani CC-SAL-25T were 56-58 mol%. A menaquinone with seven isoprene units (MK-7) was the major respiratory quinone. The fatty acid profiles were slightly different from that of B. pelovolcani CC-SAL-25T. The novel strains could be distinguished from the phylogenetically closest species B. pelovolcani CC-SAL-25T based on matrix-assisted laser desorption ionization time-of-flight mass spectra of whole cells and a range of physiological and biochemical characteristics. The data obtained suggest that strains 2C and 5CT represent a novel species of the genus Belliella, for which the name Belliella buryatensis sp. nov. is proposed. The type strain is 5CT ( = VKM B-2724T = KCTC 32194T).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkalies
  • Bacterial Typing Techniques
  • Bacteroidetes / classification*
  • Bacteroidetes / genetics
  • Bacteroidetes / isolation & purification
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Lakes / microbiology*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phylogeny*
  • Pigmentation
  • RNA, Ribosomal, 16S / genetics
  • Russia
  • Sequence Analysis, DNA
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry

Substances

  • Alkalies
  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • vitamin MK 7