TransgeneOmics--A transgenic platform for protein localization based function exploration

Methods. 2016 Mar 1;96:69-74. doi: 10.1016/j.ymeth.2015.10.005. Epub 2015 Oct 17.

Abstract

The localization of a protein is intrinsically linked to its role in the structural and functional organization of the cell. Advances in transgenic technology have streamlined the use of protein localization as a function discovery tool. Here we review the use of large genomic DNA constructs such as bacterial artificial chromosomes as a transgenic platform for systematic tag-based protein function exploration.

Keywords: BAC transgenesis; Genome engineering; Protein localization; Protein tagging; Recombineering.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / metabolism
  • Chromosomes, Artificial, Bacterial / chemistry
  • Chromosomes, Artificial, Bacterial / metabolism
  • DNA, Complementary / genetics*
  • DNA, Complementary / metabolism
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / metabolism
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism
  • Genetic Engineering / methods*
  • Genomics / methods*
  • Humans
  • Molecular Imaging / methods*
  • Staining and Labeling / methods
  • Transgenes*

Substances

  • DNA, Complementary
  • Fluorescent Dyes