Alkaline phosphatase determines polyphosphate-induced mineralization in a cell-type independent manner

J Bone Miner Metab. 2016 Nov;34(6):627-637. doi: 10.1007/s00774-015-0719-6. Epub 2015 Oct 16.

Abstract

Polyphosphate [Poly(P)] has positive effects on osteoblast mineralization; however, the underlying mechanism remains unclear. In addition, it is unknown whether Poly(P) promotes mineralization in soft tissues. We investigated this by using various cells. Poly(P) concentrations of 1 and 0.5 mg/mL yielded high levels of mineralization in ROS17/2.8 osteoblast cells. Similarly, Poly(P) induced mineralization in cell types expressing alkaline phosphatase (ALP), namely, ATDC5 and MC3T3-E1, but not in CHO, C3H10T1/2, C2C12, and 3T3-L1 cells. Furthermore, forced expression of ALP caused Poly(P)-induced mineralization in CHO cells. These results suggest that ALP determines Poly(P)-induced mineralization in a cell-type independent manner.

Keywords: Alkaline phosphatase; Mineralization; Polyphosphate.

MeSH terms

  • 3T3-L1 Cells
  • Alkaline Phosphatase / biosynthesis*
  • Alkaline Phosphatase / genetics
  • Animals
  • CHO Cells
  • Calcification, Physiologic / drug effects*
  • Calcification, Physiologic / genetics
  • Cricetinae
  • Cricetulus
  • Gene Expression Regulation, Enzymologic / drug effects*
  • Gene Expression Regulation, Enzymologic / physiology
  • Mice
  • Organ Specificity / drug effects
  • Organ Specificity / physiology
  • Osteoblasts / cytology
  • Osteoblasts / enzymology*
  • Polyphosphates / pharmacology*
  • Rats

Substances

  • Polyphosphates
  • Alkaline Phosphatase