Rhodovulum aestuarii sp. nov., isolated from a brackish water body

Int J Syst Evol Microbiol. 2016 Jan;66(1):165-171. doi: 10.1099/ijsem.0.000691. Epub 2015 Oct 15.


A yellowish brown, phototrophic, purple non-sulfur bacterium, strain JA924T, was isolated in pure culture from a brackish water sample collected from an estuary. Single cells were oval to rod-shaped, non-motile and Gram-stain-negative and had a vesicular architecture of intracellular photosynthetic membranes. Bacteriochlorophyll-a and carotenoids of the spheroidene series were present as photosynthetic pigments. Photolithoautotrophy, chemo-organoheterotrophy and photo-organoheterotrophy were the growth modes observed. Strain JA924T had complex growth requirements. Strain JA924T was mesophilic and moderately halophilic. The DNA G+C content was 64 mol% (HPLC). The major cellular fatty acids were C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and C18 : 0. The major quinone was ubiquinone-10 (Q-10). Phosphatidylglycerol, phosphatidylethanolamine, sulfolipid and an aminolipid were the main polar lipids of strain JA924T. EzTaxon-e blast searches based on the 16S rRNA gene sequence of JA924T revealed highest similarity with Rhodovulum mangrovi AK41T (98.19 %) and other members of the genus Rhodovulum ( < 95.71 %). Strain JA924T was further identified to be distantly related to Rhodovulum mangrovi AK41T ( < 29 % based on DNA-DNA hybridization and ΔTm (>5 °C). Phenotypic, chemotaxonomic and molecular differences indicate that strain JA924T represents a novel species of the genus Rhodovulum, for which the name Rhodovulum aestuarii sp. nov. is proposed. The type strain is JA924T ( = LMG 29031T = KCTC 15485T).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Bacteriochlorophyll A
  • Base Composition
  • Carotenoids / chemistry
  • DNA, Bacterial / genetics
  • Estuaries
  • Fatty Acids / chemistry
  • India
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phospholipids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Rhodovulum / classification*
  • Rhodovulum / genetics
  • Rhodovulum / isolation & purification
  • Saline Waters*
  • Sequence Analysis, DNA
  • Ubiquinone / analogs & derivatives
  • Ubiquinone / chemistry


  • Bacteriochlorophyll A
  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Ubiquinone
  • Carotenoids
  • coenzyme Q10