Electrochemical & osteoblast adhesion study of engineered TiO2 nanotubular surfaces on titanium alloys

Zia Ur Rahman; Waseem Haider; Luis Pompa; K M Deen

doi:10.1016/j.msec.2015.07.024

Electrochemical & osteoblast adhesion study of engineered TiO2 nanotubular surfaces on titanium alloys

Mater Sci Eng C Mater Biol Appl. 2016 Jan 1:58:160-8. doi: 10.1016/j.msec.2015.07.024. Epub 2015 Aug 7.

Authors

Zia Ur Rahman¹, Waseem Haider², Luis Pompa³, K M Deen⁴

Affiliations

¹ School of Engineering and Technology, Central Michigan University, Mt. Pleasant, MI, USA.
² School of Engineering and Technology, Central Michigan University, Mt. Pleasant, MI, USA. Electronic address: haide1w@cmich.edu.
³ Department of Mechanical Engineering, University of Texas-Pan American, Edinburg, TX, USA.
⁴ Department of Metallurgy & Materials Engineering, CEET, University of the Punjab, 54590 Lahore, Pakistan; Department of Materials Engineering, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.

PMID: 26478299
DOI: 10.1016/j.msec.2015.07.024

Abstract

TiO2 nanotubes were grafted on the surface of cpTi, Ti6Al4V and Ti6Al4V-ELI with the aim to provide a new podium for human pre-osteoblast cell (MC3T3) adhesion and proliferation. The surface morphology and chemistry of these alloys were examined with scanning electron microscopy and energy dispersive x-ray spectroscopy. TiO2 nanotubes were further characterized by cyclic potentiodynamic polarization tests and electrochemical impedance spectroscopy. The vertically aligned nanotubes were subjected to pre-osteoblast cell proliferation in order to better understand cell-material interaction. The study demonstrated that these cells interact differently with nanotubes of different titanium alloys. The significant acceleration in the growth rate of pre-osteoblast cell adhesion and proliferation is also witnessed. Additionally, the cytotoxicity of the leached metal ions was evaluated by using a tetrazolium-based bio-assay, MTS. Each group of data was operated for p<0.05, concluded one way ANOVA to investigate the significance difference.

Keywords: Electrochemical; Impedance spectroscopy; Nanotubes; Osteoblast; Potentiodynamic.

MeSH terms

Alloys
Cell Adhesion / drug effects*
Cell Line
Cell Proliferation / drug effects
Electrochemical Techniques
Humans
Nanotubes / chemistry*
Osteoblasts / drug effects*
Surface Properties
Titanium / chemistry*
Titanium / pharmacology

Substances

Alloys
titanium alloy (TiAl6V4)
titanium dioxide
Titanium