Effects of low-dose light-emitting-diode therapy in combination with water bath for atopic dermatitis in NC/Nga mice

Photodermatol Photoimmunol Photomed. 2016 Jan;32(1):34-43. doi: 10.1111/phpp.12220. Epub 2015 Nov 6.


Background: Light-emitting diode (LED) phototherapy and water bath therapy have beneficial effect on atopic dermatitis (AD)-like skin disease. However, not all current treatments work well and alternative therapies are need. The contribution of combination therapy with low-dose 850 nm LED and water bath was investigated on dermatophagoides farina (Df)-induced dermatitis in NC/Nga mice.

Methods: Low-dose LED (10, 15, and 20 J/cm(2) ) irradiation, water bath (36 ± 1°C) were administered separately and together to the Df-induced NC/Nga mice in acrylic jar once a day for 2 weeks.

Results: Combined therapy with low-dose LED therapy and water bath therapy significantly ameliorated the development of AD-like skin lesions. These effects were correlated with the suppression of total IgE, NO, histamine, and Th2-mediated immune responses. Furthermore, combination therapy significantly reduced the infiltration of inflammatory cells and the induction of thymic stromal lymphopoietin (TSLP) in the skin lesions. The beneficial therapeutic effects of this combination therapy might regulate by the inhibition of various immunological responses including Th2-mediated immune responses, inflammatory mediators such as IgE, histamine, and NO, as well as inflammatory cells.

Conclusions: The combination therapy of LED and water bath might be used as an efficacious, safe, and steroid-free alternative therapeutic strategy for the treatment of AD.

Keywords: NC/Nga mice; atopic dermatitis; combination therapy; light-emitting-diode phototherapy; water bath therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Baths*
  • Cytokines / immunology
  • Dermatitis, Atopic / immunology
  • Dermatitis, Atopic / pathology
  • Dermatitis, Atopic / therapy*
  • Immunoglobulin E / immunology
  • Male
  • Mice
  • Nitric Oxide / immunology
  • Phototherapy*
  • Th2 Cells / immunology
  • Th2 Cells / pathology
  • Thymic Stromal Lymphopoietin


  • Cytokines
  • Nitric Oxide
  • Immunoglobulin E
  • Thymic Stromal Lymphopoietin
  • TSLP protein, mouse