Genome-Wide Chromatin Immunoprecipitation in Candida albicans and Other Yeasts

Methods Mol Biol. 2016;1361:161-84. doi: 10.1007/978-1-4939-3079-1_10.

Abstract

Chromatin immunoprecipitation experiments are critical to investigating the interactions between DNA and a wide range of nuclear proteins within a cell or biological sample. In this chapter we outline an optimized protocol for genome-wide chromatin immunoprecipitation that has been used successfully for several distinct morphological forms of numerous yeast species, and include an optimized method for amplification of chromatin immunoprecipitated DNA samples and hybridization to a high-density oligonucleotide tiling microarray. We also provide detailed suggestions on how to analyze the complex data obtained from these experiments.

Keywords: Candida albicans; ChIP -chip; ChIP -seq; Chromatin immunoprecipitation; Yeast.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Candida albicans / genetics*
  • Chromatin / genetics
  • Chromatin Immunoprecipitation / methods*
  • DNA, Fungal / genetics*
  • Genome, Fungal
  • Nucleic Acid Hybridization / methods*

Substances

  • Chromatin
  • DNA, Fungal