Genome sequencing and annotation of Amycolatopsis azurea DSM 43854(T)

Indu Khatri; Srikrishna Subramanian; Shanmugam Mayilraj

doi:10.1016/j.gdata.2013.12.003

Genome sequencing and annotation of Amycolatopsis azurea DSM 43854(T)

Genom Data. 2014 Mar 12:2:44-5. doi: 10.1016/j.gdata.2013.12.003. eCollection 2014 Dec.

Authors

Indu Khatri¹, Srikrishna Subramanian¹, Shanmugam Mayilraj²

Affiliations

¹ Protein Science and Engineering, CSIR-Institute of Microbial Technology, Chandigarh 160036, India.
² Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Chandigarh 160036, India.

Abstract

We report the 9.2 Mb genome of the azureomycin A and B antibiotic producing strain Amycolatopsis azurea isolated from a Japanese soil sample. The draft genome of strain DSM 43854(T) consists of 9,223,451 bp with a G + C content of 69.0% and the genome contains 3 rRNA genes (5S-23S-16S) and 58 aminoacyl-tRNA synthetase genes. The homology searches revealed that the PKS gene clusters are supposed to be responsible for the biosynthesis of naptomycin, macbecin, rifamycin, mitomycin, maduropeptin enediyne, neocarzinostatin enediyne, C-1027 enediyne, calicheamicin enediyne, landomycin, simocyclinone, medermycin, granaticin, polyketomycin, teicoplanin, balhimycin, vancomycin, staurosporine, rubradirin and complestatin.

Keywords: Amycolatopsis azurea strain DSM 43854T; Illumina-HiSeq; NGS QC tool kit; Rapid Annotations using Subsystems Technology (RAST).