A pathogenesis-based transcript signature in donor-specific antibody-positive kidney transplant patients with normal biopsies

P Ó Broin; N Hayde; Y Bao; B Ye; R B Calder; G de Boccardo; M Lubetzky; M Ajaimy; J Pullman; A Colovai; E Akalin; A Golden

doi:10.1016/j.gdata.2014.10.005

A pathogenesis-based transcript signature in donor-specific antibody-positive kidney transplant patients with normal biopsies

Genom Data. 2014 Oct 12:2:357-60. doi: 10.1016/j.gdata.2014.10.005. eCollection 2014 Dec.

Authors

P Ó Broin¹, N Hayde², Y Bao³, B Ye¹, R B Calder¹, G de Boccardo⁴, M Lubetzky⁴, M Ajaimy⁴, J Pullman⁵, A Colovai³, E Akalin³, A Golden⁶

Affiliations

¹ Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA.
² Division of Pediatric Nephrology, University of Texas Health Science Center, Houston, TX, USA.
³ Montefiore-Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA.
⁴ Montefiore-Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA ; Division of Nephrology, Albert Einstein College of Medicine, Bronx, NY, USA.
⁵ Department of Pathology, Albert Einstein College of Medicine, Bronx, NY, USA.
⁶ Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA ; Department of Mathematical Sciences, Yeshiva University, New York, NY, USA.

Abstract

Affymetrix Human Gene 1.0-ST arrays were used to assess the gene expression profiles of kidney transplant patients who presented with donor-specific antibodies (DSAs) but showed normal biopsy histopathology and did not develop antibody-mediated rejection (AMR). Biopsy and whole-blood profiles for these DSA-positive, AMR-negative (DSA +/AMR-) patients were compared to both DSA-positive, AMR-positive (DSA +/AMR +) patients as well as DSA-negative (DSA -) controls. While individual gene expression changes across sample groups were relatively subtle, gene-set enrichment analysis using previously identified pathogenesis-based transcripts (PBTs) identified a clear molecular signature involving increased rejection-associated transcripts in AMR - patients. Results from this study have been published in Kidney International (Hayde et al., 2014 [1]) and the associated data have been deposited in the GEO archive and are accessible via the following link: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE50084.

Keywords: Antibody-mediated rejection; Donor-specific antibodies; Gene expression; Kidney; Transplant.