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. 2015 Mar 12;4:50-3.
doi: 10.1016/j.gdata.2015.03.001. eCollection 2015 Jun.

Gene Expression Profiling During Intensive Cardiovascular Lifestyle Modification: Relationships With Vascular Function and Weight Loss

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Free PMC article

Gene Expression Profiling During Intensive Cardiovascular Lifestyle Modification: Relationships With Vascular Function and Weight Loss

Heather L Blackburn et al. Genom Data. .
Free PMC article

Abstract

Heart disease and related sequelae are a leading cause of death and healthcare expenditure throughout the world. Although many patients opt for surgical interventions, lifestyle modification programs focusing on nutrition and exercise have shown substantial health benefits and are becoming increasing popular. We conducted a year-long lifestyle modification program to mediate cardiovascular risk through traditional risk factors and to investigate how molecular changes, if present, may contribute to long-term risk reduction. Here we describe the lifestyle intervention, including clinical and molecular data collected, and provide details of the experimental methods and quality control parameters for the gene expression data generated from participants and non-intervention controls. Our findings suggest successful and sustained modulation of gene expression through healthy lifestyle changes may have beneficial effects on vascular health that cannot be discerned from traditional risk factor profiles. The data are deposited in the Gene Expression Omnibus, series GSE46097 and GSE66175.

Keywords: Cardiovascular disease; Gene expression; Lifestyle modification; Microarray; Weight loss.

Figures

Fig. 1
Fig. 1
Intensity graph showing the RMA normalized log2 intensity for each array. The median intensity curve is highlighted in green.
Fig. 2
Fig. 2
Three-dimensional scatter plot representing a Principal Component Analysis of all expression arrays colored by laboratory technician.
Fig. 3
Fig. 3
Histogram (top panel) and scatter plot (bottom panel) showing the percentage of probes on each array yielding detectable expression (percent present calls). The median of the percent present calls is represented by the dashed green line and statistical limits [± 3.5 × STD (mean absolute deviation)] by the dashed red lines.

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