In Arabidopsis, various cell type-specific whole-genome expression analyses have been conducted. However, the vast majority of these were performed with cellular RNA from root tissues or other easily accessible cell types . Nuclear RNA was neglected for a long time as not being representative for transcriptomic studies. In recent years, however, there have been reports describing the validity of nuclear RNA for these types of studies [2,3]. Here we describe the generation, quality assessment and analysis of nuclear transcriptomic data from Arabidopsis embryos published by Slane et al. (2014) . Comparison of nuclear with cellular gene expression demonstrated the usefulness of nuclear transcriptomics.
Keywords: Arabidopsis thaliana; Gene expression; Microarray; Nuclear and cellular transcriptome; Pro-embryo and suspensor transcriptome.