Isolation of chitinolytic Clostridium sp. NCR from Mehsani buffalo rumen, its genomic analysis and potential role in rumen

Neelam M Nathani; Srinivas M Duggirala; Chandra Shekar M; Ramesh K Kothari; Chaitanya G Joshi

doi:10.1016/j.gdata.2015.05.017

Isolation of chitinolytic Clostridium sp. NCR from Mehsani buffalo rumen, its genomic analysis and potential role in rumen

Genom Data. 2015 May 30:5:109-11. doi: 10.1016/j.gdata.2015.05.017. eCollection 2015 Sep.

Authors

Neelam M Nathani¹, Srinivas M Duggirala², Chandra Shekar M³, Ramesh K Kothari⁴, Chaitanya G Joshi³

Affiliations

¹ Department of Animal Biotechnology, Anand Agricultural University, Anand, Gujarat, India ; Department of Biosciences, Saurashtra University, Rajkot, Gujarat, India.
² Department of Microbiology, Gujarat Vidyapeeth, Sadra, Gujarat, India.
³ Department of Animal Biotechnology, Anand Agricultural University, Anand, Gujarat, India.
⁴ Department of Biosciences, Saurashtra University, Rajkot, Gujarat, India.

Abstract

Genomic analysis of Clostridium sp. NCR, an anaerobic Gram positive bacterium which was isolated from rumen fluid of Mehsani breed of buffalo revealed presence of various environmental gene tags (EGTs) involved in pathways for utilizing a wide range of substrates. Here we report the sequence of this rumen isolate, its whole genome sequence has been deposited in DDBJ/EMBL/GenBank under the accession number JQHY00000000. The genome comprises of a 3.62-Mb draft genome with a G + C content of 28.10%, which encodes a total of 3126 proteins. Functional analysis provides information about the microbe's role in maintaining host homeostasis and its fiber degradation potential.

Keywords: Chitinolytic; Clostridium sp. NCR; Genome sequence; Rumen microflora.