The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes

Science. 1989 Mar 17;243(4897):1464-6. doi: 10.1126/science.2648569.


T lymphocyte chemotactic factor (TCF) was purified to homogeneity from the conditioned media of phytohemagglutinin-stimulated human blood mononuclear leukocytes by a sequence of chromatography procedures. The amino-terminal amino acid sequence of the purified TCF showed identity with neutrophil-activating protein (NAP-1). Both TCF and recombinant NAP-1 (rNAP-1) were chemotactic for neutrophils and T lymphocytes in vitro supporting the identity of TCF with NAP-1. Injection of rNAP-1 into lymphatic drainage areas of lymph nodes in Fisher rats caused accelerated emigration of only lymphocytes in high endothelial venules. Intradermal injection of rNAP-1 caused dose-dependent accumulation of neutrophils and lymphocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chemotactic Factors / isolation & purification*
  • Chemotaxis, Leukocyte*
  • Interleukin-8
  • Peptides / isolation & purification*
  • Rats
  • T-Lymphocytes / immunology*


  • Chemotactic Factors
  • Interleukin-8
  • Peptides