Modification of additive effect between vitamins and ETS on childhood asthma risk according to GSTP1 polymorphism: a cross -sectional study

BMC Pulm Med. 2015 Oct 22:15:125. doi: 10.1186/s12890-015-0093-0.

Abstract

Background: Asthma is characterized by airway inflammation, and bronchial airways are particularly susceptible to oxidant-induced tissue damage.

Objective: To investigate the effect of dietary antioxidant intake and environmental tobacco smoke (ETS) on the risk of childhood asthma according to genotypes susceptible to airway diseases.

Methods: This cross-sectional study included 1124 elementary school children aged 7-12 years old. Asthma symptoms and smoking history were measured using the International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire. Intake of vitamin A (including retinol and β-carotene), C, and E was measured by a semi-quantitative food frequency questionnaire (FFQ). GSTP1 polymorphisms were genotyped from peripheral blood samples.

Results: ETS was significantly associated with presence of asthma symptoms (adjusted odds ratio [aOR], 2.48; 95 % confidence interval [CI], 1.29-4.76) and diagnosis (aOR, 1.91; 95 % CI, 1.19-3.06). Dietary antioxidant intake was not associated with asthma symptoms, although ETS plus low vitamin A intake showed a significant positive association with asthma diagnosis (aOR, 2.23; 95 % CI, 1.10-4.54). Children with AA at nucleotide 1695 in GSTP1 who had been exposed to ETS and a low vitamin A intake have an increased risk of asthma diagnosis (aOR, 4.44; 95 % CI,1.58-12.52) compared with children who had not been exposed to the two risk factors. However, ETS exposure and low vitamin A intake did not significantly increase odds of asthma diagnosis in children with AG or GG genotypes.

Conclusion: Low vitamin A intake and ETS exposure may increase oxidative stress and thereby risk for childhood asthma. These relationships may be modified by gene susceptibility alleles of GSTP1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascorbic Acid
  • Asthma / epidemiology*
  • Asthma / genetics
  • Child
  • Cross-Sectional Studies
  • Diet / statistics & numerical data*
  • Female
  • Gene-Environment Interaction*
  • Genetic Predisposition to Disease
  • Glutathione S-Transferase pi / genetics*
  • Humans
  • Male
  • Odds Ratio
  • Polymorphism, Genetic
  • Tobacco Smoke Pollution / statistics & numerical data*
  • Vitamin A
  • Vitamin E
  • Vitamins*
  • beta Carotene

Substances

  • Tobacco Smoke Pollution
  • Vitamins
  • beta Carotene
  • Vitamin A
  • Vitamin E
  • GSTP1 protein, human
  • Glutathione S-Transferase pi
  • Ascorbic Acid