Acute effects of grayanotoxin in rhododendron honey on kidney functions in rats

Environ Sci Pollut Res Int. 2016 Feb;23(4):3300-9. doi: 10.1007/s11356-015-5534-z. Epub 2015 Oct 21.

Abstract

The aim of the study is to evaluate the acute biochemical and histological changes in rat kidneys after treatment with grayanotoxin (GTX) of rhododendron honey (RH). A total of 60 Sprague-Dawley female rats were divided into five groups of 12 rats each, one being a control group (group 1) and group 2 was treated with 0.015 mg/kg/bw of GTX standard preparation via intraperitoneal injection. Groups 3, 4, and 5 were given RH at doses of 0.1, 0.5, and 2.5 g/kg/bw, respectively, via oral gavage. Compared to the control group, significant increases were observed in glucose, blood urea nitrogen (BUN), and creatinine levels of the GTX-injected groups after 1 h. However, in low dose RH group, such an increase was not observed and had a normal appearance histologically. Therefore, low dose (1 g/kg/bw) of RH produces no acute adverse effects on renal functions of rats.

Keywords: Biochemical parameters; Gryanotoxin; Mad honey poisoning; Rat; Rhododendron honey.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Urea Nitrogen
  • Creatinine / blood
  • Diterpenes / toxicity*
  • Female
  • Honey / analysis*
  • Kidney / drug effects*
  • Kidney / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Rhododendron / chemistry
  • Rhododendron / toxicity*

Substances

  • Diterpenes
  • Creatinine
  • grayanotoxin I