Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton

Cell. 1989 Apr 21;57(2):233-42. doi: 10.1016/0092-8674(89)90961-6.


The yeast tropomyosin gene, designated TPM1, is present in a single copy per haploid genome and encodes a protein with a predicted molecular weight of 23.5 kd. The protein sequence is homologous to higher cell tropomyosins, including the characteristic hydrophobic-hydrophilic pseudoheptapeptide repeats. Indirect immunofluorescence microscopy reveals that tropomyosin is localized with actin cables in wild-type cells. Disruption of TPM1 is not lethal, but results in a reduced growth rate and disappearance of actin cables. Strains carrying the conditional actin mutation act1-2 also lack actin cables; overexpression of tropomyosin in these strains partially restores actin cables. These results strongly suggest that tropomyosin interacts with F actin in vivo and may play an important role in assembling or stabilizing actin cables in yeast.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / metabolism*
  • Base Sequence
  • Cytoskeleton / analysis
  • Cytoskeleton / metabolism*
  • Gene Expression Regulation
  • Genes*
  • Genes, Lethal
  • Molecular Sequence Data
  • Saccharomyces cerevisiae
  • Tropomyosin / analysis
  • Tropomyosin / genetics*


  • Actins
  • Tropomyosin

Associated data

  • GENBANK/M25501