Comparison of Gold Nanoparticle Conjugated Secondary Antibody with Non-Gold Secondary Antibody in an ELISA Kit Model

Monoclon Antib Immunodiagn Immunother. 2015 Oct;34(5):366-70. doi: 10.1089/mab.2015.0021.

Abstract

In this study, gold nanoparticles (AuNPs) were used as carriers of the signaling anti-chicken antibody peroxidase in comparison with anti-chicken antibody peroxidase without gold nanoparticle in a commercial avian influenza kit. AuNPs enhanced the absorbance and shortened the assay time. AuNPs act as a carrier of many enzymes and multiply the effect of enzyme when reacting with substrate. They amplify optical signal, while keeping low background signals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / immunology*
  • Chickens / immunology
  • Chickens / virology
  • Drug Carriers / administration & dosage
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Gold / administration & dosage*
  • Influenza in Birds / immunology
  • Metal Nanoparticles / administration & dosage*
  • Peroxidase / immunology

Substances

  • Antibodies
  • Drug Carriers
  • Gold
  • Peroxidase