Use of gene replacement to construct Escherichia coli strains carrying mutations in two genes required for stability of multicopy plasmids

J Bacteriol. 1989 Apr;171(4):2241-3. doi: 10.1128/jb.171.4.2241-2243.1989.


Escherichia coli mutants completely defective in ColE1 cer-mediated site-specific recombination have been mapped to two genes, xerA and xerB. In this study, xerA xerB double mutants were constructed by gene replacement with a lambda dv plasmid and were shown to be both viable and defective in ColE1 site-specific recombination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Replication
  • Escherichia coli / genetics*
  • Genes, Bacterial*
  • Genetic Engineering / methods
  • Plasmids*
  • Recombination, Genetic*