Spirosoma pulveris sp. nov., a bacterium isolated from a dust sample collected at Chungnam province, South Korea

J Microbiol. 2015 Nov;53(11):750-5. doi: 10.1007/s12275-015-5263-5. Epub 2015 Oct 28.

Abstract

Strain JSH 5-14(T), a Gram-negative, non-motile, and curved rod-shaped bacterium, was isolated from a dust sample collected at Nonsan, Chungnam province, South Korea, and was characterized to determine its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence of strain JSH 5-14(T) revealed that it belongs to the genus Spirosoma, family Cytophagaceae, class Cytophagia. The highest degree of sequence similarities of strain JSH 5-14(T) were found with Spirosoma liguale DSM 74(T) (97.8%) and Spirosoma endophyticum EX 36(T) (96.2%). The predominant fatty acids were summed feature 3 (composed of C16:1 ω7c/C16:1 ω6c) and C16:1 ω5c. The major polar lipid was phosphatidylethanolamine, and the predominant respiratory quinone was MK-7. Based on the phylogenetic, chemotaxonomic, and phenotypic data, we propose the strain JSH 5-14(T) (=KCTC 42550(T) =JCM 30688(T) =KEMB 9004-165(T)) should be classified as a type strain of a novel species, for which the name Spirosoma pulveris sp. nov., is proposed.

Keywords: Cytophagaceae; Cytophagia; Spirosoma; taxonomy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Cluster Analysis
  • Cytophagaceae / chemistry
  • Cytophagaceae / classification*
  • Cytophagaceae / genetics
  • Cytophagaceae / isolation & purification*
  • Dust*
  • Fatty Acids / analysis
  • Phosphatidylethanolamines / chemistry
  • Phospholipids / analysis
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Sequence Analysis, DNA
  • Soil Microbiology*

Substances

  • Dust
  • Fatty Acids
  • Phosphatidylethanolamines
  • Phospholipids
  • RNA, Ribosomal, 16S
  • phosphatidylethanolamine