Design of Enzyme-Encapsulated Protein Containers by In Vivo Crystal Engineering

Adv Mater. 2015 Dec 22;27(48):7951-6. doi: 10.1002/adma.201503827. Epub 2015 Oct 27.


Crystalline protein assemblies of polyhedra crystal (PhC) can be utilized as solid enzyme containers for long-term storage of enzymes with retention of their enzymatic activity. The enzymes can be released from the crystals at the optimum pH for the enzymatic activity by dissolution of the crystals using in vivo crystal engineering.

Keywords: crystal engineering; enzyme encapsulation; polyhedra; protein crystals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Capsules
  • Crystallization
  • Models, Molecular
  • Protein Engineering / methods*
  • Protein Kinase C / genetics*
  • Protein Structure, Secondary
  • Sf9 Cells
  • Spodoptera
  • Viral Structural Proteins / chemistry*
  • Viral Structural Proteins / genetics*


  • Capsules
  • Viral Structural Proteins
  • Protein Kinase C