GALNT7, a target of miR-494, participates in the oncogenesis of nasopharyngeal carcinoma

Tumour Biol. 2016 Apr;37(4):4559-67. doi: 10.1007/s13277-015-4281-6. Epub 2015 Oct 27.

Abstract

GalNAc-transferase-7 (GALNT7) is essential for the regulation of cell proliferation and has been implicated in tumorigenesis. However, the role of GALNT7 in the development and progression of nasopharyngeal carcinoma (NPC) remains unclear. Our previous study showed that GALNT7 was a putative target of miR-494, which was confirmed by luciferase reporter assay. In the present study, we demonstrated that in vitro knockdown of GALNT7 significantly inhibited the proliferation, colony formation, migration, and invasion of NPC-derived cells. In vivo tumorigenicity assay showed that miR-494 and GALNT7-small interfering RNA (siRNA) reduced tumor growth in nude mice. Taken together, our results provided new evidence for an oncogenic role of GALNT7 in NPC.

Keywords: GALNT7; Nasopharyngeal carcinoma; Oncogenicity; Tumor suppressor; miR-494.

MeSH terms

  • Animals
  • Apoptosis
  • Carcinogenesis / genetics*
  • Carcinogenesis / metabolism
  • Carcinoma
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Male
  • Mice, Nude
  • MicroRNAs / genetics*
  • N-Acetylgalactosaminyltransferases / genetics*
  • N-Acetylgalactosaminyltransferases / metabolism
  • Nasopharyngeal Carcinoma
  • Nasopharyngeal Neoplasms / genetics*
  • Nasopharyngeal Neoplasms / metabolism
  • Nasopharyngeal Neoplasms / pathology
  • Neoplasm Transplantation
  • Polypeptide N-acetylgalactosaminyltransferase
  • RNA Interference
  • Tumor Burden

Substances

  • MIRN494 microRNA, human
  • MicroRNAs
  • N-Acetylgalactosaminyltransferases