Optogenetic Mapping of Intracortical Circuits Originating From Semilunar Cells in the Piriform Cortex

Cereb Cortex. 2017 Jan 1;27(1):589-601. doi: 10.1093/cercor/bhv258.

Abstract

Despite its comparatively simple trilaminar architecture, the primary olfactory (piriform) cortex of mammals is capable of performing sophisticated sensory processing, an ability that is thought to depend critically on its extensive associational (intracortical) excitatory circuits. Here, we used a novel transgenic mouse model and optogenetics to measure the connectivity of associational circuits that originate in semilunar (SL) cells in layer 2a of the anterior piriform cortex (aPC). We generated a mouse line (48L) in which channelrhodopsin-2 (ChR) could be selectively expressed in a subset of SL cells. Light-evoked excitatory postsynaptic currents (EPSCs) could be evoked in superficial pyramidal cells (17.4% of n = 86 neurons) and deep pyramidal cells (33.3%, n = 9) in the aPC, but never in ChR- SL cells (0%, n = 34). Thus, SL cells monosynaptically excite pyramidal cells, but not other SL cells. Light-evoked EPSCs were also selectively elicited in 3 classes of GABAergic interneurons in layer 3 of the aPC. Our results show that SL cells are specialized for providing feedforward excitation of specific classes of neurons in the aPC, confirming that SL cells comprise a functionally distinctive input layer.

Keywords: 48L; anatomy; channelrhodopsin; interneuron; olfactory cortex.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Mapping
  • Channelrhodopsins / genetics
  • Channelrhodopsins / metabolism
  • Excitatory Postsynaptic Potentials
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Confocal
  • Neural Pathways / cytology
  • Neural Pathways / physiology
  • Neurons / cytology
  • Neurons / physiology*
  • Optogenetics
  • Patch-Clamp Techniques
  • Piriform Cortex / cytology
  • Piriform Cortex / physiology*
  • Tissue Culture Techniques
  • gamma-Aminobutyric Acid / metabolism

Substances

  • Channelrhodopsins
  • gamma-Aminobutyric Acid