Active Hexose-correlated Compound Down-regulates Heat Shock Factor 1, a Transcription Factor for HSP27, in Gemcitabine-resistant Human Pancreatic Cancer Cells

Anticancer Res. 2015 Nov;35(11):6063-7.

Abstract

Background: Active hexose-correlated compound (AHCC) is an extract of a basidiomycete mushroom that enhances the therapeutic effects and reduces the side-effects of chemotherapy. Our previous studies demonstrated that heat-shock protein 27 (HSP27) was involved in gemcitabine-resistance of pancreatic cancer cells and it was down-regulated by AHCC-treatment. However, how AHCC down-regulated HSP27 is unknown. In the present study, we focused on two transcription factors reported to induce HSP27, heat shock factor 1 (HSF1) and high-mobility group box 1 (HMGB1) and investigated the effect of AHCC on their expression.

Materials and methods: KLM1-R cells were treated with AHCC and the protein expression of HSF1 and HMGB1 were analyzed by western blotting.

Results: The protein expression of HSF1 in KLM1-R was down-regulated by AHCC treatment. On the other hand, the protein expression of HMGB1 was not reduced in KLM1-R cells after AHCC treatment.

Conclusion: The possibility that AHCC down-regulated HSP27 through down-regulation of the HSF1, was herein shown.

Keywords: AHCC; HMGB1; HSF1; HSP27; gemcitabine; pancreatic cancer.

MeSH terms

  • Antimetabolites, Antineoplastic / pharmacology
  • Blotting, Western
  • DNA-Binding Proteins / antagonists & inhibitors*
  • DNA-Binding Proteins / metabolism
  • Deoxycytidine / analogs & derivatives*
  • Deoxycytidine / pharmacology
  • Drug Resistance, Neoplasm / drug effects*
  • HMGB1 Protein / antagonists & inhibitors*
  • HMGB1 Protein / metabolism
  • HSP27 Heat-Shock Proteins / antagonists & inhibitors*
  • HSP27 Heat-Shock Proteins / metabolism
  • Heat Shock Transcription Factors
  • Heat-Shock Proteins
  • Humans
  • Molecular Chaperones
  • Pancreatic Neoplasms / drug therapy*
  • Pancreatic Neoplasms / metabolism*
  • Pancreatic Neoplasms / pathology
  • Polysaccharides / pharmacology*
  • Transcription Factors / antagonists & inhibitors*
  • Transcription Factors / metabolism
  • Tumor Cells, Cultured

Substances

  • Antimetabolites, Antineoplastic
  • DNA-Binding Proteins
  • HMGB1 Protein
  • HMGB1 protein, human
  • HSF1 protein, human
  • HSP27 Heat-Shock Proteins
  • HSPB1 protein, human
  • Heat Shock Transcription Factors
  • Heat-Shock Proteins
  • Molecular Chaperones
  • Polysaccharides
  • Transcription Factors
  • Deoxycytidine
  • gemcitabine
  • Active Hexose Correlated Compound