Extended-spectrum β-lactamase (ESBL) detection directly from urine samples with the rapid isothermal amplification-based eazyplex® SuperBug CRE assay: Proof of concept

J Microbiol Methods. 2015 Dec;119:203-5. doi: 10.1016/j.mimet.2015.10.015. Epub 2015 Oct 23.

Abstract

A commercially available assay (eazyplex® SuperBug CRE) detecting the most common carbapenemase and ESBL types was evaluated directly on 50 urine samples. Eazyplex® correctly detected ESBL-encoding genes in all 30 urine samples with confirmed ESBL production (sensitivity 100%). Two specimens showed invalid and one specimen false-positive results (specificity 97.9%).

Keywords: ESBL; Isothermal amplification; Proof of concept; Rapid detection; Urine samples.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / urine*
  • Enterobacteriaceae / enzymology*
  • Enterobacteriaceae / genetics
  • Enterobacteriaceae / isolation & purification
  • Enterobacteriaceae Infections / microbiology
  • Enterobacteriaceae Infections / urine*
  • Humans
  • Nucleic Acid Amplification Techniques / economics
  • Nucleic Acid Amplification Techniques / methods*
  • Reagent Kits, Diagnostic
  • beta-Lactamases / genetics
  • beta-Lactamases / urine*

Substances

  • Bacterial Proteins
  • Reagent Kits, Diagnostic
  • beta-Lactamases
  • carbapenemase