The systematic analysis of protein-lipid interactions comes of age

Nat Rev Mol Cell Biol. 2015 Dec;16(12):753-61. doi: 10.1038/nrm4080. Epub 2015 Oct 28.


Lipids tailor membrane identities and function as molecular hubs in all cellular processes. However, the ways in which lipids modulate protein function and structure are poorly understood and still require systematic investigation. In this Innovation article, we summarize pioneering technologies, including lipid-overlay assays, lipid pull-down assays, affinity-purification lipidomics and the liposome microarray-based assay (LiMA), that will enable protein-lipid interactions to be deciphered on a systems level. We discuss how these technologies can be applied to the charting of system-wide networks and to the development of new pharmaceutical strategies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cell Membrane / metabolism*
  • Humans
  • Lipid Metabolism / physiology
  • Lipids / chemistry*
  • Lipoproteins / chemistry*
  • Liposomes / chemistry*
  • Microarray Analysis / methods*
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Proteins / chemistry*


  • Lipids
  • Lipoproteins
  • Liposomes
  • Proteins