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. 2015 Aug 1;3:82-4.
doi: 10.7150/jgen.12886. eCollection 2015.

Draft Genome Sequences of Two Species of "Difficult-to-Identify" Human-Pathogenic Corynebacteria: Implications for Better Identification Tests

Free PMC article

Draft Genome Sequences of Two Species of "Difficult-to-Identify" Human-Pathogenic Corynebacteria: Implications for Better Identification Tests

Luis G C Pacheco et al. J Genomics. .
Free PMC article


Non-diphtheriae Corynebacterium species have been increasingly recognized as the causative agents of infections in humans. Differential identification of these bacteria in the clinical microbiology laboratory by the most commonly used biochemical tests is challenging, and normally requires additional molecular methods. Herein, we present the annotated draft genome sequences of two isolates of "difficult-to-identify" human-pathogenic corynebacterial species: C. xerosis and C. minutissimum. The genome sequences of ca. 2.7 Mbp, with a mean number of 2,580 protein encoding genes, were also compared with the publicly available genome sequences of strains of C. amycolatum and C. striatum. These results will aid the exploration of novel biochemical reactions to improve existing identification tests as well as the development of more accurate molecular identification methods through detection of species-specific target genes for isolate's identification or drug susceptibility profiling.

Keywords: Biochemical tests; Corynebacterium spp.; Emerging pathogens; Molecular identification.

Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.


Figure 1
Figure 1
Venn diagram demonstrating the numbers of coding sequences shared by the four Corynebacterium species analyzed in this study.

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    1. Martins C, Faria L, Souza M, Camello T, Velasco E, Hirata R Jr, Thuler L, Mattos-Guaraldi A. Microbiological and host features associated with corynebacteriosis in cancer patients: a five-year study. Mem Inst Oswaldo Cruz. 2009;104:905–913. - PubMed
    1. Bernard K. The genus corynebacterium and other medically relevant coryneform-like bacteria. J Clin Microbiol. 2012;50:3152–3158. - PMC - PubMed
    1. Ramana KV, Vikram G, PadmaWali P, K A, Rao M, Rao SD, MS RM, CH V, Rao R. Non Diphtheritic Corynebacteria (NDC) and Their Clinical Significance: Clinical Microbiologist's Perspective. Am J Epidemiol Infect Dis. 2014;2:83–87.
    1. Shin JY, Lee WK, Seo YH, Park YS. Postoperative Abdominal Infection Caused by Corynebacterium minutissimum. Infect Chemother. 2014;46:261–263. - PMC - PubMed
    1. Funke G, Lawson PA, Bernard KA, Collins MD. Most Corynebacterium xerosis strains identified in the routine clinical laboratory correspond to Corynebacterium amycolatum. J Clin Microbiol. 1996;34:1124–1128. - PMC - PubMed