Fission Yeast Cell Cycle Synchronization Methods

Methods Mol Biol. 2016;1369:293-308. doi: 10.1007/978-1-4939-3145-3_20.

Abstract

Fission yeast cells can be synchronized by cell cycle arrest and release or by size selection. Cell cycle arrest synchronization is based on the block and release of temperature-sensitive cell cycle mutants or treatment with drugs. The most widely used approaches are cdc10-129 for G1; hydroxyurea (HU) for early S-phase; cdc25-22 for G2, and nda3-KM311 for mitosis. Cells can also be synchronized by size selection using centrifugal elutriation or a lactose gradient. Here we describe the methods most commonly used to synchronize fission yeast cells.

Keywords: Block and release; Centrifugal elutriation; Fission yeast; G1-phase; G2-phase; HU; Lactose gradient; Mitosis; S-phase; Schizosaccharomyces pombe; Synchronization; cdc10-129; cdc25-22; nda3-KM311.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle* / drug effects
  • Cell Cycle* / genetics
  • Hydroxyurea / pharmacology
  • Mitosis / drug effects
  • Mitosis / genetics
  • Mutation
  • Schizosaccharomyces / physiology*

Substances

  • Hydroxyurea