Cell-based screens and phenomics with fission yeast

Crit Rev Biochem Mol Biol. 2016;51(2):86-95. doi: 10.3109/10409238.2015.1103205. Epub 2015 Nov 2.


Next-generation sequencing approaches have considerably advanced our understanding of genome function and regulation. However, the knowledge of gene function and complex cellular processes remains a challenge and bottleneck in biological research. Phenomics is a rapidly emerging area, which seeks to rigorously characterize all phenotypes associated with genes or gene variants. Such high-throughput phenotyping under different conditions can be a potent approach toward gene function. The fission yeast Schizosaccharomyces pombe (S. pombe) is a proven eukaryotic model organism that is increasingly used for genomewide screens and phenomic assays. In this review, we highlight current large-scale, cell-based approaches used with S. pombe, including computational colony-growth measurements, genetic interaction screens, parallel profiling using barcodes, microscopy-based cell profiling, metabolomic methods and transposon mutagenesis. These diverse methods are starting to offer rich insights into the relationship between genotypes and phenotypes.

Keywords: Drug screen; functional profiling; metabolomics; microbial growth; mutant screen.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acids / metabolism
  • DNA Transposable Elements
  • Genome, Viral*
  • Metabolomics
  • Models, Biological
  • Mutagenesis
  • Phenotype*
  • Schizosaccharomyces / classification
  • Schizosaccharomyces / genetics*


  • Amino Acids
  • DNA Transposable Elements