Composite Wound Dressings of Pectin and Gelatin With Aloe Vera and Curcumin as Bioactive Agents

Int J Biol Macromol. 2016 Jan;82:104-13. doi: 10.1016/j.ijbiomac.2015.10.087. Epub 2015 Nov 1.


Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices. The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix. A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings. However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings. The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells. OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation. On the other hand, OP-Gel-Curcumin and OP-Gel-Aloe Curcumin seemed to have induced apoptosis in NIH3T3 cells. In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice. OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days. Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention. Histological examination revealed that an ordered collagen formation and neovascularization could be observed along with migration of nuclei. Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.

Keywords: Biocomposite; Oxidized pectin; Wound healing.

MeSH terms

  • Aloe / chemistry*
  • Animals
  • Anti-Infective Agents / chemistry
  • Anti-Infective Agents / pharmacology
  • Antioxidants / chemistry
  • Antioxidants / pharmacology
  • Biocompatible Materials / chemistry*
  • Biocompatible Materials / pharmacology
  • Cell Survival
  • Curcumin / chemistry*
  • Curcumin / pharmacology*
  • Drug Liberation
  • Gelatin / chemistry*
  • Male
  • Mice
  • Microbial Sensitivity Tests
  • NIH 3T3 Cells
  • Pectins / chemistry*
  • Wound Healing / drug effects*


  • Anti-Infective Agents
  • Antioxidants
  • Biocompatible Materials
  • Pectins
  • Gelatin
  • Curcumin