Trypan Blue Exclusion Test of Cell Viability

Curr Protoc Immunol. 2015 Nov 2;111:A3.B.1-A3.B.3. doi: 10.1002/0471142735.ima03bs111.


The protocol described in this appendix allows for light microscopic quantitation of cell viability. Cells are suspended in PBS containing trypan blue and then examined to determine the percentage of cells that have clear cytoplasm (viable cells) versus cells that have blue cytoplasm (nonviable cells).

Keywords: cell viability; dye exclusion; trypan blue.

MeSH terms

  • Animals
  • Cell Survival*
  • Cells, Cultured
  • Cytoplasm / metabolism*
  • Humans
  • Microscopy
  • Staining and Labeling / methods*
  • Trypan Blue / chemistry*


  • Trypan Blue