Standardized Multi-Color Flow Cytometry and Computational Biomarker Discovery

Methods Mol Biol. 2016;1371:225-38. doi: 10.1007/978-1-4939-3139-2_15.


Multi-color flow cytometry has become a valuable and highly informative tool for diagnosis and therapeutic monitoring of patients with immune deficiencies or inflammatory disorders. However, the method complexity and error-prone conventional manual data analysis often result in a high variability between different analysts and research laboratories. Here, we provide strategies and guidelines aiming at a more standardized multi-color flow cytometric staining and unsupervised data analysis for whole blood patient samples.

Keywords: Data analysis; Flow cytometry; Immune monitoring; Standardization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / metabolism*
  • Computational Biology / methods*
  • Computational Biology / standards
  • Flow Cytometry / methods*
  • Flow Cytometry / standards
  • Humans
  • Immune System / cytology*
  • Immune System / physiology*
  • Immunophenotyping / methods*
  • Immunophenotyping / standards
  • Leukocytes / immunology
  • Leukocytes / metabolism


  • Biomarkers