Synthesis, Characterization and Fluorescence Properties of Zn(II) and Cu(II) Complexes: DNA Binding Study of Zn(II) Complex

J Fluoresc. 2016 Jan;26(1):333-44. doi: 10.1007/s10895-015-1719-6. Epub 2015 Nov 4.

Abstract

Zinc(II) and copper(II) complexes containing Schiff base, 2- methoxy-6((E)-(phenylimino) methyl) phenol ligand (HL) were synthesized and characterized by elemental analysis, IR, NMR, and single crystal X-ray diffraction technique. The fluorescence properties and quantum yield of zinc complex were studied. Our data showed that Zn complex could bind to DNA grooves with Kb = 10(4) M(-1). Moreover, Zn complex could successfully be used in staining of DNA following agarose gel electrophoresis. MTT assay showed that Zn complex was not cytotoxic in MCF-7 cell line. Here, we introduce a newly synthesized fluorescence probe that can be used for single and double stranded DNA detection in both solution and agarose gels.

Keywords: Copper; DNA; Fluorescence; Interaction; Schiff base; Zinc.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Binding Sites
  • Copper / chemistry*
  • Crystallography, X-Ray
  • DNA / analysis*
  • DNA / chemistry*
  • Electrophoresis, Agar Gel
  • Fluorescence*
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Humans
  • MCF-7 Cells
  • Models, Molecular
  • Organometallic Compounds / chemical synthesis
  • Organometallic Compounds / chemistry*
  • Spectrometry, Fluorescence
  • Zinc / chemistry*

Substances

  • Fluorescent Dyes
  • Organometallic Compounds
  • Copper
  • DNA
  • Zinc