Activation of interleukin-1 beta by a co-induced protease

FEBS Lett. 1989 Apr 24;247(2):386-90. doi: 10.1016/0014-5793(89)81376-6.

Abstract

The proteolytic generation of mature interleukin-1 beta (IL-1 beta) from its inactive precursor does not proceed by a conventional pathway for hormonal processing. Pro-IL-1 beta is found dispersed in the cytoplasm, and there are no basic amino acid residues or other commonly recognized processing sites adjoining the mature N-terminus. Processing appears to occur during release of the hormone. In the present study, we have identified a specific protease that generates mature IL-1 beta from the precursor. This enzyme is co-induced with the hormone, and it differs in its cleavage specificity and inhibitor sensitivity from all known proteases.

MeSH terms

  • Blotting, Western
  • Cell Line
  • Cytoplasm / metabolism
  • Ethylmaleimide / pharmacology
  • Interleukin-1 / physiology*
  • Iodoacetates / pharmacology
  • Iodoacetic Acid
  • Monocytes / enzymology
  • Peptide Hydrolases / isolation & purification
  • Peptide Hydrolases / metabolism*
  • Protease Inhibitors / pharmacology
  • Protein Precursors / metabolism*
  • Recombinant Proteins / metabolism
  • Substrate Specificity

Substances

  • Interleukin-1
  • Iodoacetates
  • Protease Inhibitors
  • Protein Precursors
  • Recombinant Proteins
  • Peptide Hydrolases
  • Ethylmaleimide
  • Iodoacetic Acid