A long natural-antisense RNA is accumulated in the conidia of Aspergillus oryzae

Biosci Biotechnol Biochem. 2016;80(2):386-98. doi: 10.1080/09168451.2015.1101333. Epub 2015 Nov 5.

Abstract

Analysis of expressed sequence tag libraries from various culture conditions revealed the existence of conidia-specific transcripts assembled to putative conidiation-specific reductase gene (csrA) in Aspergillus oryzae. However, the all transcripts were transcribed with opposite direction to the gene csrA. The sequence analysis of the transcript revealed that the RNA overlapped mRNA of csrA with 3'-end, and did not code protein longer than 60 amino acid residues. We designated the transcript Conidia Specific Long Natural-antisense RNA (CSLNR). The real-time PCR analysis demonstrated that the CSLNR is conidia-specific transcript, which cannot be transcribed in the absence of brlA, and the amount of CSLNR was much more than that of the transcript from csrA in conidia. Furthermore, the csrA deletion, also lacking coding region of CSLNR in A. oryzae reduced the number of conidia. Overexpression of CsrA demonstrated the inhibition of growth and conidiation, while CSLNR did not affect conidiation.

Keywords: Aspergillus oryzae; CSLNR; long natural antisense RNA.

MeSH terms

  • Aspergillus oryzae / genetics*
  • Aspergillus oryzae / metabolism
  • Base Sequence
  • Exons
  • Expressed Sequence Tags
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Deletion
  • Gene Expression Regulation, Fungal*
  • Introns
  • Molecular Sequence Data
  • RNA, Antisense / genetics*
  • RNA, Antisense / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Real-Time Polymerase Chain Reaction
  • Spores, Fungal / genetics*
  • Spores, Fungal / metabolism
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcription, Genetic

Substances

  • Fungal Proteins
  • RNA, Antisense
  • RNA, Messenger
  • Transcription Factors