Electroactive polyurethane/siloxane derived from castor oil as a versatile cardiac patch, part I: Synthesis, characterization, and myoblast proliferation and differentiation

J Biomed Mater Res A. 2016 Mar;104(3):775-787. doi: 10.1002/jbm.a.35612. Epub 2015 Dec 21.


Tissue-engineered cardiac patch aims at regenerating an infarcted heart by improving cardiac function and providing mechanical support to the diseased myocardium. In order to take advantages of electroactivity, a new synthetic method was developed for the introduction of an electroactive oligoaniline into the backbone of prepared patches. For this purpose, a series of electroactive polyurethane/siloxane films containing aniline tetramer (AT) was prepared through sol-gel reaction of trimethoxysilane functional intermediate polyurethane prepolymers made from castor oil and poly(ethylene glycol). Physicochemical, mechanical, and electrical conductivity of samples were evaluated and the recorded results were correlated to their structural characteristics. The optimized films were proved to be biodegradable and have tensile properties suitable for cardiac patch application. The embedded AT moieties in the backbone of the prepared samples preserved their electroactivity with the electrical conductivity in the range of 10-4 S/cm. The prepared films were compatible with proliferation of C2C12 and had potential for enhancing myotube formation even without external electrical stimulation. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 775-787, 2016.

Keywords: C2C12 myoblast; aniline tetramer; cardiac patch; castor oil; polyurethane.

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry
  • Castor Oil / chemistry*
  • Cell Differentiation / drug effects*
  • Cell Line
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Elasticity
  • Electric Conductivity*
  • Electrochemical Techniques
  • Hydrophobic and Hydrophilic Interactions
  • Materials Testing
  • Mice
  • Myoblasts / cytology*
  • Myoblasts / drug effects
  • Myoblasts / metabolism
  • Polyurethanes / chemical synthesis*
  • Polyurethanes / chemistry
  • Polyurethanes / pharmacology*
  • Siloxanes / chemical synthesis*
  • Siloxanes / chemistry
  • Siloxanes / pharmacology*
  • Spectroscopy, Fourier Transform Infrared
  • Tensile Strength
  • Tissue Engineering / methods
  • Viscosity


  • Biocompatible Materials
  • Polyurethanes
  • Siloxanes
  • Castor Oil