Modeling and measurement of vesicle pools at the cone ribbon synapse: Changes in release probability are solely responsible for voltage-dependent changes in release

Synapse. 2016 Jan;70(1):1-14. doi: 10.1002/syn.21871. Epub 2015 Nov 6.

Abstract

Postsynaptic responses are a product of quantal amplitude (Q), size of the releasable vesicle pool (N), and release probability (P). Voltage-dependent changes in presynaptic Ca(2+) entry alter postsynaptic responses primarily by changing P but have also been shown to influence N. With simultaneous whole cell recordings from cone photoreceptors and horizontal cells in tiger salamander retinal slices, we measured N and P at cone ribbon synapses by using a train of depolarizing pulses to stimulate release and deplete the pool. We developed an analytical model that calculates the total pool size contributing to release under different stimulus conditions by taking into account the prior history of release and empirically determined properties of replenishment. The model provided a formula that calculates vesicle pool size from measurements of the initial postsynaptic response and limiting rate of release evoked by a train of pulses, the fraction of release sites available for replenishment, and the time constant for replenishment. Results of the model showed that weak and strong depolarizing stimuli evoked release with differing probabilities but the same size vesicle pool. Enhancing intraterminal Ca(2+) spread by lowering Ca(2+) buffering or applying BayK8644 did not increase PSCs evoked with strong test steps, showing there is a fixed upper limit to pool size. Together, these results suggest that light-evoked changes in cone membrane potential alter synaptic release solely by changing release probability.

Keywords: cone photoreceptor; modeling; ribbon synapse; vesicle pools.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / pharmacology
  • Ambystoma
  • Animals
  • Calcium / metabolism
  • Calcium Channel Agonists / pharmacology
  • Calcium Channels / metabolism
  • Female
  • Kinetics
  • Male
  • Membrane Potentials / drug effects
  • Membrane Potentials / physiology*
  • Models, Neurological
  • Patch-Clamp Techniques
  • Probability
  • Retina / drug effects
  • Retina / physiology*
  • Retinal Cone Photoreceptor Cells / drug effects
  • Retinal Cone Photoreceptor Cells / physiology*
  • Retinal Horizontal Cells / drug effects
  • Retinal Horizontal Cells / physiology*
  • Synapses / drug effects
  • Synapses / physiology*
  • Synaptic Vesicles / drug effects
  • Synaptic Vesicles / metabolism*
  • Tissue Culture Techniques

Substances

  • Calcium Channel Agonists
  • Calcium Channels
  • 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
  • Calcium