Flow cytometry in hematological nonmalignant disorders

Int J Lab Hematol. 2016 Feb;38(1):5-16. doi: 10.1111/ijlh.12438. Epub 2015 Nov 6.


Multiparameter flow cytometry (MFC) has become an integral part of the diagnosis and classification of hematological malignancies. However, several nonmalignant or premalignant disorders may benefit from this technology in hematology laboratories. This review provides information on the normal immunophenotypic characteristics of peripheral blood leukocyte subsets and their modifications in several clinical conditions. The usefulness of MFC and the specific markers that can be investigated in hyperlymphocytosis, infection, hypereosinophilia, paroxysmal nocturnal hemoglobinuria, and large granular lymphocyte disorders is described. Mention is also made of the developments of MFC for analyses of red blood cells or platelets.

Keywords: Flow cytometry; erythrocytes; leukocytes; nonmalignant disorders; peripheral blood.

Publication types

  • Review

MeSH terms

  • Biomarkers
  • Blood Cell Count / methods
  • Blood Platelets / metabolism
  • Erythrocytes / metabolism
  • Flow Cytometry* / methods
  • Hematologic Diseases / blood*
  • Hematologic Diseases / diagnosis*
  • Hematologic Neoplasms / blood
  • Hematologic Neoplasms / diagnosis
  • Humans
  • Immunophenotyping / methods
  • Leukocytes / metabolism
  • Phenotype


  • Biomarkers