Neutralization of Japanese Encephalitis Virus by heme-induced broadly reactive human monoclonal antibody

Sci Rep. 2015 Nov 6:5:16248. doi: 10.1038/srep16248.


Geographical expansion and re-emerging new genotypes of the Japanese encephalitis virus (JEV) require the development of novel therapeutic approaches. Here, we studied a non-conventional approach for antibody therapy and show that, upon exposure to heme, a fraction of natural human immunoglobulins acquires high-affinity reactivity with the antigenic domain-III of JEV E glycoprotein. These JEV-reactive antibodies exhibited neutralizing activity against recently dominant JEV genotypes. This study opens new therapeutic options for Japanese encephalitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Cell Line
  • Cricetinae
  • Encephalitis Virus, Japanese / immunology*
  • Encephalitis Virus, Japanese / pathogenicity
  • Heme / administration & dosage*
  • Humans
  • Immunoglobulin G / immunology
  • Kinetics
  • Neutralization Tests*
  • Thermodynamics
  • Virulence


  • Antibodies, Monoclonal
  • Immunoglobulin G
  • Heme