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. 2016 Apr;38:125-129.
doi: 10.1016/j.anaerobe.2015.10.013. Epub 2015 Nov 3.

Complete Genome Sequences and Analysis of the Fusobacterium Nucleatum Subspecies Animalis 7-1 Bacteriophage ɸFunu1 and ɸFunu2

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Free PMC article

Complete Genome Sequences and Analysis of the Fusobacterium Nucleatum Subspecies Animalis 7-1 Bacteriophage ɸFunu1 and ɸFunu2

Kyla Cochrane et al. Anaerobe. .
Free PMC article

Abstract

Fusobacterium nucleatum is a strictly anaerobic, Gram negative bacterial species that has been associated with dental infections, pre-term labor, appendicitis, inflammatory bowel disease, and, more recently, colorectal cancer. The species is unusual in its phenotypic and genotypic heterogeneity, with some strains demonstrating a more virulent phenotype than others; however, as yet the genetic basis for these differences is not understood. Bacteriophage are known to contribute to the virulence phenotype of several bacterial species. In this work, we set out to characterize the bacteriophage associated with F. nucleatum subsp. animalis strain 7-1, a highly invasive isolate from the human gastrointestinal tract. As well, we used computational approaches to predict and compare bacteriophage signatures across available sequenced F. nucleatum genomes.

Figures

Figure 1
Figure 1
Results of clustering analysis carried out for predicted phage sequences. Strains are arranged according to previously published phylogeny (28). F. nucleatum strains 11_3_2, 4_1_13 and F0401 all contain similar phage sequences that cluster with ɸFunu1. ɸFunu2 is unique among the strains examined.
Figure 2
Figure 2
Representative electron micrograph images of phage particles isolated and purified from F.nucleatum strain 7_1. Panels A) ɸFunu1; and B) ɸFunu2. Arrow in panel A indicates a putative phage tail structure. Note the absence of a tail structure for the representative virion head imaged in panel B.

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