Isolation and analysis of discreet human prostate cellular populations

Differentiation. Apr-Jun 2016;91(4-5):139-51. doi: 10.1016/j.diff.2015.10.013. Epub 2015 Nov 3.

Abstract

The use of lineage tracing in transgenic mouse models has revealed an abundance of subcellular phenotypes responsible for maintaining prostate homeostasis. The ability to use fresh human tissues to examine the hypotheses generated by these mouse experiments has been greatly enhanced by technical advances in tissue processing, flow cytometry and cell culture. We describe in detail the optimization of protocols for each of these areas to facilitate research on solving human prostate diseases through the analysis of human tissue.

Publication types

  • Review
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / genetics*
  • Cell Separation / methods*
  • Epithelial Cells / cytology*
  • Flow Cytometry
  • Humans
  • Male
  • Mice
  • Mice, Transgenic
  • Prostate / cytology*
  • Prostate / growth & development