Upregulated CDK16 Expression in Serous Epithelial Ovarian Cancer Cells

Med Sci Monit. 2015 Nov 7;21:3409-14. doi: 10.12659/msm.894990.


Background: As CDK-16 has been shown to be upregulated in several transformed cancer lines, we hypothesized that the cyclin-dependent kinase 16 (CDK-16) may be upregulated in serous epithelial ovarian cancer (EOC) cells. Therefore, we comparatively examined the mRNA and protein expression of CDK-16 in samples resected from serous EOC patients and normal controls.

Material and methods: Tissue samples were collected from 70 serous EOC patients and 40 normal controls. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was conducted to assess mRNA expression. CDK-16 protein expression was assessed by semi-quantitative immunohistochemical staining. Differences in mRNA and protein expression between serous EOC cells and normal tissue cells were tested with the Kruskal-Wallis test and analysis of variance (ANOVA).

Results: Both CDK-16 mRNA and protein expression were significantly higher in serous EOC tumor cells as compared to normal control ovarian cells (p<0.01). Although there was no significant correlation between CDK-16 mRNA expression and serous EOC stage (p=0.0794), there was a significant correlation between CDK-16 mRNA expression and serous EOC grade (p<0.0001). Moreover, there were significant correlations between CDK-16 protein expression and serous EOC stage (p<0.0001) and grade (p<0.0001).

Conclusions: CDK-16 upregulation in serous EOC cells may represent a negative feedback loop to promote ovarian cell differentiation in malignantly-transformed serous EOC cells. Further in-depth investigation on CDK-16's role in serous EOC is needed.

MeSH terms

  • Adult
  • Analysis of Variance
  • Carcinoma, Ovarian Epithelial
  • Case-Control Studies
  • Cyclin-Dependent Kinases / metabolism*
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Immunohistochemistry
  • Middle Aged
  • Neoplasms, Glandular and Epithelial / metabolism*
  • Ovarian Neoplasms / metabolism*
  • Ovary / metabolism
  • RNA, Messenger / metabolism
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sample Size
  • Up-Regulation


  • RNA, Messenger
  • Cyclin-Dependent Kinases
  • PCTAIRE-1 protein kinase