Two-Photon Lithography of 3D Nanocomposite Piezoelectric Scaffolds for Cell Stimulation

ACS Appl Mater Interfaces. 2015 Nov 25;7(46):25574-9. doi: 10.1021/acsami.5b08764. Epub 2015 Nov 10.


In this letter, we report on the fabrication, the characterization, and the in vitro testing of structures suitable for cell culturing, prepared through two-photon polymerization of a nanocomposite resist. More in details, commercially available Ormocomp has been doped with piezoelectric barium titanate nanoparticles, and bioinspired 3D structures resembling trabeculae of sponge bone have been fabricated. After an extensive characterization, preliminary in vitro testing demonstrated that both the topographical and the piezoelectric cues of these scaffolds are able to enhance the differentiation process of human SaOS-2 cells.

Keywords: barium titanate nanoparticles; bone tissue engineering; direct laser writing; piezoelectric stimulation; two-photon lithography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation
  • Cell Line, Tumor
  • Electricity*
  • Humans
  • Imaging, Three-Dimensional*
  • Nanocomposites / chemistry*
  • Nanocomposites / ultrastructure
  • Osteogenesis
  • Photons*
  • Printing / methods*
  • Tissue Scaffolds / chemistry*