Lycopene treatment against loss of bone mass, microarchitecture and strength in relation to regulatory mechanisms in a postmenopausal osteoporosis model

Bone. 2016 Feb;83:127-140. doi: 10.1016/j.bone.2015.10.017. Epub 2015 Nov 5.

Abstract

Lycopene supplementation decreases oxidative stress and exhibits beneficial effects on bone health, but the mechanisms through which it alters bone metabolism in vivo remain unclear. The present study aims to evaluate the effects of lycopene treatment on postmenopausal osteoporosis. Six-month-old female Wistar rats (n=264) were sham-operated (SHAM) or ovariectomized (OVX). The SHAM group received oral vehicle only and the OVX rats were randomized into five groups receiving oral daily lycopene treatment (mg/kg body weight per day): 0 OVX (control), 15 OVX, 30 OVX, and 45 OVX, and one group receiving alendronate (ALN) (2μg/kg body weight per day), for 12weeks. Bone densitometry measurements, bone turnover markers, biomechanical testing, and histomorphometric analysis were conducted. Micro computed tomography was also used to evaluate changes in microarchitecture. Lycopene treatment suppressed the OVX-induced increase in bone turnover, as indicated by changes in biomarkers of bone metabolism: serum osteocalcin (s-OC), serum N-terminal propeptide of type 1 collagen (s-PINP), serum crosslinked carboxyterminal telopeptides (s-CTX-1), and urinary deoxypyridinoline (u-DPD). Significant improvement in OVX-induced loss of bone mass, bone strength, and microarchitectural deterioration was observed in lycopene-treated OVX animals. These effects were observed mainly at sites rich in trabecular bone, with less effect in cortical bone. Lycopene treatment down-regulated osteoclast differentiation concurrent with up-regulating osteoblast together with glutathione peroxidase (GPx) catalase (CAT) and superoxide dismutase (SOD) activities. These findings demonstrate that lycopene treatment in OVX rats primarily suppressed bone turnover to restore bone strength and microarchitecture.

Keywords: Bone mass and microarchitecture; Bone turnover; Lycopene; Osteoclastogenesis; Ovariectomy; Oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Absorptiometry, Photon
  • Animals
  • Biomarkers / blood
  • Biomechanical Phenomena / drug effects
  • Body Weight / drug effects
  • Bone Density / drug effects
  • Bone Remodeling / drug effects
  • Bone Resorption / blood
  • Bone Resorption / diagnostic imaging
  • Bone Resorption / drug therapy*
  • Bone Resorption / physiopathology*
  • Bone and Bones / diagnostic imaging
  • Bone and Bones / drug effects
  • Bone and Bones / pathology*
  • Bone and Bones / physiopathology*
  • Carotenoids / blood
  • Carotenoids / pharmacology
  • Carotenoids / therapeutic use*
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / blood
  • Diaphyses / diagnostic imaging
  • Diaphyses / drug effects
  • Diaphyses / physiopathology
  • Disease Models, Animal
  • Enzymes / blood
  • Female
  • Femur / diagnostic imaging
  • Femur / drug effects
  • Femur / physiopathology
  • Hormones / blood
  • Humans
  • Humerus / diagnostic imaging
  • Humerus / drug effects
  • Humerus / physiopathology
  • Lumbar Vertebrae / diagnostic imaging
  • Lumbar Vertebrae / drug effects
  • Lumbar Vertebrae / physiopathology
  • Lycopene
  • Minerals / blood
  • Organ Size / drug effects
  • Osteoporosis, Postmenopausal / blood
  • Osteoporosis, Postmenopausal / drug therapy*
  • Osteoporosis, Postmenopausal / physiopathology*
  • Rats, Wistar
  • Tibia / diagnostic imaging
  • Tibia / drug effects
  • Tibia / physiopathology
  • Uterus / drug effects
  • Uterus / pathology
  • X-Ray Microtomography

Substances

  • Biomarkers
  • Enzymes
  • Hormones
  • Minerals
  • Carotenoids
  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine
  • Lycopene