Correlation Between Arteriosclerosis and Periodontal Condition Assessed by Lactoferrin and α1-Antitrypsin Levels in Gingival Crevicular Fluid

Int Heart J. 2015;56(6):639-43. doi: 10.1536/ihj.15-218. Epub 2015 Nov 9.


Patients with periodontal disease exhibit exacerbated atherosclerosis, aortic stiffness, or vascular endothelial dysfunction. However, in a recent scientific statement, the American Heart Association noted that neither has periodontal disease been proven to cause atherosclerotic vascular disease nor has the treatment of periodontal disease been proven to prevent atherosclerotic vascular disease. Therefore, the aim of the present study was to examine the correlation between periodontal condition and arteriosclerosis in patients with coronary artery disease (CAD), which is usually accompanied by systemic arteriosclerosis.We measured levels of gingival crevicular fluid lactoferrin (GCF-Lf) and α1-antitrypsin (GCF-AT) in 72 patients (67 ± 8 years, 56 men) with CAD. Furthermore, we evaluated the maximum intima-media thickness (max IMT) and plaque score of the carotid arteries as well as brachial-ankle pulse wave velocity (baPWV) and flow-mediated dilation (FMD) of the brachial artery, each of which is a parameter for determining arteriosclerosis status. The average level of GCF-Lf was 0.29 ± 0.36 µg/mL and that of GCF-AT was 0.31 ± 0.66 µg/mL, with significant correlation between the two (r = 0.701, P < 0.001). No significant difference in GCF-Lf and GCF-AT levels was observed between patients with single-, double-, and triple-vessel CAD. There were no significant correlations between the arteriosclerosis parameters (ie, max IMT, plaque score, baPWV, and FMD) and GCF-Lf or GCF-AT.No correlation between the GCF biomarkers and the severity of arteriosclerosis was detected. This result may suggest that worsening of the periodontal condition assessed by GCF biomarkers is not a major potential risk factor for arteriosclerosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Ankle Brachial Index / methods
  • Atherosclerosis* / diagnosis
  • Atherosclerosis* / epidemiology
  • Atherosclerosis* / metabolism
  • Atherosclerosis* / physiopathology
  • Biomarkers / metabolism
  • Carotid Intima-Media Thickness
  • Coronary Artery Disease* / diagnosis
  • Coronary Artery Disease* / epidemiology
  • Female
  • Humans
  • Lactoferrin / metabolism*
  • Male
  • Middle Aged
  • Periodontal Diseases* / epidemiology
  • Periodontal Diseases* / metabolism
  • Pulse Wave Analysis / methods
  • Random Allocation
  • Risk Assessment / methods
  • Risk Factors
  • Severity of Illness Index
  • Statistics as Topic
  • alpha 1-Antitrypsin / metabolism*


  • Biomarkers
  • LTF protein, human
  • alpha 1-Antitrypsin
  • Lactoferrin