Dietary Fiber-Induced Improvement in Glucose Metabolism Is Associated with Increased Abundance of Prevotella

Cell Metab. 2015 Dec 1;22(6):971-82. doi: 10.1016/j.cmet.2015.10.001. Epub 2015 Nov 6.


The gut microbiota plays an important role in human health by interacting with host diet, but there is substantial inter-individual variation in the response to diet. Here we compared the gut microbiota composition of healthy subjects who exhibited improved glucose metabolism following 3-day consumption of barley kernel-based bread (BKB) with those who responded least to this dietary intervention. The Prevotella/Bacteroides ratio was higher in responders than non-responders after BKB. Metagenomic analysis showed that the gut microbiota of responders was enriched in Prevotella copri and had increased potential to ferment complex polysaccharides after BKB. Finally, germ-free mice transplanted with microbiota from responder human donors exhibited improved glucose metabolism and increased abundance of Prevotella and liver glycogen content compared with germ-free mice that received non-responder microbiota. Our findings indicate that Prevotella plays a role in the BKB-induced improvement in glucose metabolism observed in certain individuals, potentially by promoting increased glycogen storage.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Animals
  • Bacteroides / genetics
  • Bacteroides / growth & development
  • Bacteroides / physiology
  • Blood Glucose / analysis
  • Cross-Over Studies
  • Dietary Fiber / pharmacology*
  • Feces / microbiology
  • Female
  • Glucose / metabolism*
  • Glycogen / metabolism
  • Humans
  • Hydrogen / metabolism
  • Insulin / blood
  • Intestines / microbiology
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Microbiota / drug effects*
  • Middle Aged
  • Prevotella / genetics
  • Prevotella / growth & development*
  • Prevotella / physiology
  • RNA, Ribosomal, 16S / chemistry
  • RNA, Ribosomal, 16S / metabolism


  • Blood Glucose
  • Dietary Fiber
  • Insulin
  • RNA, Ribosomal, 16S
  • Hydrogen
  • Glycogen
  • Glucose